FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_F01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_F01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880711
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG77610.41266308327010365No Hit
TTATAATACTGTGACCACCACCAGTTTCGAGAAATACTACCTTTTGCCTC69430.3691688941044105No Hit
GTCTATAAGATATCCAGGTAAATATATTGACAAGACAAATGGAAATGAGC39940.21236649331024277No Hit
GTGCTGGAGAACAGCCCCCACTGCGACACCATGGTGGACAACAACCTGCG34860.18535543206797855No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG33450.17785826743183827No Hit
GTGTATACTCTGCCTGAACGTAATAATAGCAGATATATTGGTGAACAAGG32080.17057378831729064No Hit
GAGTATACACTGGGTATTTTAAAATATGGATGATGGATTGGTATGCGTAT29000.154197003154658No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA25720.1367567903840622No Hit
TCTTGATACAACCCATTTTGCCCAGGAAACTTGCTTCTGGCATCCGTTAT25150.13372602170136721No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC23680.125909828782838No Hit
GTCCTAAGCGAGGACTAAGAGAGAATGAAGGCCACTGTCCCAGGTCACCC22090.11745557929953086No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG19460.10347150625481534No Hit
ATAATACTGTGACCACCACCAGTTTCGAGAAATACTACCTTTTGCCTCAA18830.10012170928973138No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)