FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5941
Sequences flagged as poor quality0
Sequence length151
%GC67

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTA64410.83992593839421No Hit
AGCGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGC3195.369466419794647No Hit
GGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGC2293.8545699377209224No Hit
CTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACG1632.7436458508668573No Hit
GCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGG1392.3396734556471976No Hit
CTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCC1232.0703585255007573No Hit
GGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCC1212.036694159232453No Hit
CCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACG1111.8683723278909274No Hit
GGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGC1091.8347079616226225No Hit
GAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGA981.649553947146945No Hit
GCGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCT841.41390338326881No Hit
TCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCT801.3465746507321998No Hit
GCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGG661.110924086854065No Hit
GCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGG570.9594344386466925No Hit
CTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCA550.9257700723783876No Hit
GGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTG550.9257700723783876No Hit
GCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAAC550.9257700723783876No Hit
GTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGC520.87527352297593No Hit
GCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGG520.87527352297593No Hit
CCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACG480.8079447904393199No Hit
GGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGA450.7574482410368625No Hit
GTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGC430.7237838747685574No Hit
GGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGC410.6901195085002525No Hit
GCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTG400.6732873253660999No Hit
TGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGG380.639622959097795No Hit
CCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCT370.6227907759636424No Hit
CAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGA370.6227907759636424No Hit
CCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTC370.6227907759636424No Hit
CCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGC350.5891264096953375No Hit
GAGCGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAG340.572294226561185No Hit
CACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTG330.5554620434270325No Hit
GAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCG330.5554620434270325No Hit
AGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGT320.53862986029288No Hit
CCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGC320.53862986029288No Hit
GCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTT320.53862986029288No Hit
CTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTT310.5217976771587275No Hit
GTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGGCAG310.5217976771587275No Hit
GCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAGAC310.5217976771587275No Hit
ACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCT300.504965494024575No Hit
GGGAAGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGG280.47130112775627No Hit
CCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGAC280.47130112775627No Hit
GGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGG270.45446894462211745No Hit
CGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAG260.437636761487965No Hit
GAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAG250.42080457835381246No Hit
CTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGC250.42080457835381246No Hit
ACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACC250.42080457835381246No Hit
CCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGC250.42080457835381246No Hit
GTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCC240.40397239521965994No Hit
GAGCAAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAG240.40397239521965994No Hit
CCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCC220.370308028951355No Hit
GGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACA220.370308028951355No Hit
CCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCC210.3534758458172025No Hit
GAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGT190.3198114795488975No Hit
CTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTC190.3198114795488975No Hit
GCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCT190.3198114795488975No Hit
CACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCA190.3198114795488975No Hit
ACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACAC180.30297929641474497No Hit
GCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACC180.30297929641474497No Hit
GAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAGACCGAAG180.30297929641474497No Hit
GAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAG180.30297929641474497No Hit
GCAAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCT180.30297929641474497No Hit
CCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCCTGC170.2861471132805925No Hit
CGAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTG160.26931493014644No Hit
ACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGC160.26931493014644No Hit
GCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAG160.26931493014644No Hit
AAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGT160.26931493014644No Hit
TCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTG160.26931493014644No Hit
GGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGT150.2524827470122875No Hit
GCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGA150.2524827470122875No Hit
CCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACTGCCACGCTCCTCG150.2524827470122875No Hit
CGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAA150.2524827470122875No Hit
CAGCAGGGGTGGGGGAAGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGG140.235650563878135No Hit
AAAGCAGGCTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTCACCC140.235650563878135No Hit
AGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGGCA130.2188183807439825No Hit
GCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTT130.2188183807439825No Hit
CCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGGCCGCTCCTA130.2188183807439825No Hit
GCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTA130.2188183807439825No Hit
TCGCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGC120.20198619760982997No Hit
AGCAAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGC120.20198619760982997No Hit
TGCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGG120.20198619760982997No Hit
AGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGC120.20198619760982997No Hit
ACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCCCC120.20198619760982997No Hit
CGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCG110.1851540144756775No Hit
GCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGG110.1851540144756775No Hit
AAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGC110.1851540144756775No Hit
GTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGT100.16832183134152498No Hit
AGCGAAAGCAGGCTTCGGGCCCCCGCTCCTTCTCCCTGCCCCTCGACCTC100.16832183134152498No Hit
GAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGG100.16832183134152498No Hit
GCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGA100.16832183134152498No Hit
TCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCG100.16832183134152498No Hit
GCGAGGAGCGTGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGG100.16832183134152498No Hit
GCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGC100.16832183134152498No Hit
AGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAG90.15148964820737248No Hit
CTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCT90.15148964820737248No Hit
CTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCCT90.15148964820737248No Hit
GCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCCGCCCTGCCC90.15148964820737248No Hit
GGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCG90.15148964820737248No Hit
GGAAGCGCAGCTGGTAGGAGCGGCCCGGGAGGAGCGTGGCGAGGAGCGTG80.13465746507322No Hit
CTCCTTCTCCCTGCCCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTT80.13465746507322No Hit
GTCGGCAACAGCAGGGGTGGGGGAAGTAGAGACCGAAGCCAGGAGGGTGG80.13465746507322No Hit
CACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCCCCCCTG80.13465746507322No Hit
GGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCG80.13465746507322No Hit
CTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTC80.13465746507322No Hit
GAAAGCAGGGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGG80.13465746507322No Hit
TCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCCTCTCC80.13465746507322No Hit
GAAGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGG80.13465746507322No Hit
GGTGGGGGAAGTAGAGACCGAAGCCAGGAGGGTGGCGGGCTGGGTGAGGT70.1178252819390675No Hit
GCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGCC70.1178252819390675No Hit
GCCACGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGC70.1178252819390675No Hit
CGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTCACT60.10099309880491499No Hit
GCCGACACGCCGCCGTTCACTGCCACGCTCCTCGCCACGCTCCTCCCGGG60.10099309880491499No Hit
GTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAGACCGA60.10099309880491499No Hit
CGCCACCCTCCTGGCTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCG60.10099309880491499No Hit
CGCTCCTCCCGGGCCGCTCCTACCAGCTGCGCTTCCTCTTCCGCGCTCGC60.10099309880491499No Hit
AGCCAGGAGGGTGGCGGGCTGGGTGAGGTCGAGGGGCAGGGAGAAGGAGC60.10099309880491499No Hit
GGCAGGGCGGAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCG60.10099309880491499No Hit
CTTCGGTCTCTACTTCCCCCACCCCTGCTGTTGCCGACACGCCGCCGTTC60.10099309880491499No Hit
GAGAGGCGAGCGCGGAAGAGGAAGCGCAGCTGGTAGGAGCGGCCCGGGAG60.10099309880491499No Hit
TGGCAGTGAACGGCGGCGTGTCGGCAACAGCAGGGGTGGGGGAAGTAGAG60.10099309880491499No Hit
CCCTCGACCTCACCCAGCCCGCCACCCTCCTGGCTTCGGTCTCTACTTCC60.10099309880491499No Hit
CGGCCCGGGAGGAGCGTGGCGAGGAGCGTGGCAGTGAACGGCGGCGTGTC60.10099309880491499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)