FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_D10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_D10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290572
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC124960.5455405898613971No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG121950.5323997673943451No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT100550.438973321947531No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT96420.42094289112064587No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC83540.3647123949825633No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA82400.359735472187733No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA72500.31651482686420684No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT68000.29686907898987674No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC64240.28045396521043653No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT55060.24037663954680316No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT53470.23343514196453985No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC52510.22924404908468277No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT50190.21911557462502815No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG48210.21047144556032293No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT47700.20824492746789885No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG47210.20610572381047182No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT46160.20152171597312812No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG43860.19148055594847052No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA42660.18624168984864917No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC41830.18261814079627273No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA41280.1802169938338546No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA39110.17074337763667766No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT37290.1627977640519486No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC35830.15642381029716595No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA35400.15454654994472997No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA35010.15284391846228804No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT34500.15061740036986393No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC34500.15061740036986393No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT34150.14908939775741606No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG33960.14825991062494434No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA32600.1423225290451468No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG31600.13795680729529566No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA31540.13769486399030462No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT31070.13564297476787457No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC30700.13402765772042965No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC30420.13280525563047132No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG29910.13057873753804727No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC29450.12857050553311575No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG28980.12651861631068573No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT28930.12630033022319315No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC28760.12555815752571847No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT28330.12368089717328248No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAG28000.1222402089958316No Hit
ACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTC27910.12184729403834502No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG27800.12136706464586139No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT27270.11905323211844028No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT27050.11809277333347304No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT26940.11761254394098943No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGC26840.1171759717660043No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT26720.11665208515602217No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA26380.11516773976107278No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG26140.11411996654110851No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC25690.1121553917536755No Hit
ACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCC25470.11119493296870826No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA24880.1086191571362961No Hit
TGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCG24690.10778967000382438No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACT24430.10665458234886308No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT24140.10538852304140625No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC24110.10525755138891071No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT24020.10486463643142412No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT23910.1043844070389405No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC23870.10420977816894646No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC23850.10412246373394943No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAAC23820.10399149208145389No Hit
GTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA23510.10263811833900005No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC23440.10233251781651047No Hit
CTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCC23180.10119743016154915No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC23010.10045525746407448No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC22970.10028062859408042No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT22960.10023697137658191No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT22940.10014965694158491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)