FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963069
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAACTGTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAATCT49910.5182390877496836No Hit
GCCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAG49850.5176160794294073No Hit
GTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATAATGAAATGTT49420.5131511864674286No Hit
CTGTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAAT49180.5106591531863242No Hit
CATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCA48650.5051559130238851No Hit
GTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAA45860.47618602613104566No Hit
CTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCAGAGCTGCTGGTTC45530.472759480369527No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT45260.46995594292828446No Hit
AAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAG45010.46736007492713405No Hit
GAAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGA44950.4667370666068578No Hit
GACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGA42400.4402592129951229No Hit
CTTCTACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTC41790.43392529507231564No Hit
CTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCA41510.43101792291102714No Hit
ATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGTTCT40710.4227111453073456No Hit
GGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATG40310.4185577565055048No Hit
GTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCAGAGCT40200.41741557458499856No Hit
CCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGA38810.4029825484986018No Hit
GACTATTACAGTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAG38660.40142502769791155No Hit
CTCCTGGAACTAGTTCACCTCTTGACCTTCACAGCTACAAGAGCTTGAAC36290.3768161990470049No Hit
GTGTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGCAGCCAG35550.3691324297635995No Hit
CCACTAAAGCCTCCAGAAGGAACATAGTCCTGCGGACACCTTGACTTGAA34800.36134482576014804No Hit
GGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAAT34310.3562569244778931No Hit
GTATATTAGCTTTCTGACTATTACAGTGCTAATCACGGCAGAGCAAACAC33790.35085751903550005No Hit
CTCCATAACTGTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAAT32680.3393318651103919No Hit
GAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAATGGGGTGTTTGC32630.3388126915101618No Hit
AAAGCAGGCAGCCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATC32000.3322711041472626No Hit
CATAAGCATATGTCACTGTGGCCCAGGCCTCGCCCATGGGTCCAGAGCCA31570.32780621118528375No Hit
GTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAA30110.31264634205856484No Hit
GGGAAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATA29500.30631242413575765No Hit
GAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGCTG29270.3039242255746992No Hit
ATCTTCTAGTTTCCCAAGTTGGAAGGGTGTTTTTAAGGGTAGGGGAGTCA28970.30080918397331863No Hit
GTTCTGATGATGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCA27880.2894911994883025No Hit
CCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGC26790.27817321500328634No Hit
GTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCACTGTGGACATCT26590.27609652060236595No Hit
AAAGCAGGCAGAGGGCTTAGCTGCTGAAACTGTGGAGCAGGGAGAGAGCA25600.26581688331781006No Hit
CTATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAACCACTATAA25560.265401544437626No Hit
GACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGA25530.2650900402774879No Hit
GTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGC25510.26488237083739585No Hit
GTGGACATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCACTA25440.26415552779707374No Hit
CTATAAGGCATTTCATGCAAAAAGACACGTTCAACATTTTTGCTTTCCCA24650.2559525849134382No Hit
CTACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGA23570.24473843514846808No Hit
AGCGAAAGCAGGGAAATGGAACTTGCCCATGGGTAGTGTCCTTCTGGGCC23430.24328474906782382No Hit
GCTACAAGAGCTTGAACATTTCATTATTAGCTTCAACCAGGTATATTAGC22880.2375738394652927No Hit
GTGTAGAAGATGAAATGAGAACCAGCAGCTCTGCTCTCTGCATTATTTTC22600.23466646730400417No Hit
GATTAGCACTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATA22520.233835789543636No Hit
GACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCACCTCTTGACCT22230.23082458266230144No Hit
CATCAGGACCCGTGGCCCCTTCTGGCCTCTGGTGTGAACCAGGGAGTGTG22090.22937089658165719No Hit
GGCTAATACTCTATAACGGAAACTAAGGTTCCTAATCAGGGCCTCTTGCT22050.2289555577014731No Hit
CCTCTTGACCTTCACAGCTACAAGAGCTTGAACATTTCATTATTAGCTTC21890.2272942021807368No Hit
CTTCACCACCATTCTCAAAAGACAACATCAGGACCCGTGGCCCCTTCTGG21350.22168712729825174No Hit
ACTGTGGACATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCA21040.21846825097682512No Hit
GCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAG20880.21680689545608883No Hit
AGCTTGAACATTTCATTATTAGCTTCAACCAGGTATATTAGCTTTCTGAC20790.21587238297567463No Hit
GAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGA20330.21109598585355777No Hit
GCTAATATACCTGGTTGAAGCTAATAATGAAATGTTCAAGCTCTTGTAGC20190.20964229977291346No Hit
TTACAGTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAGATGAA19920.20683876233167095No Hit
ATTTGATACTATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAAC19900.20663109289157888No Hit
GGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATG19830.20590424985125677No Hit
CCCTTAGCCCTCTCTCTAGTCCTCGTCCCCGCCGAGTTAAAGTGTCTCCA19800.2055927456911187No Hit
ATAATGCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAATG19730.20486590265079654No Hit
CCACTAAATCTCCACTAAAGCCTCCAGAAGGAACATAGTCCTGCGGACAC19260.19998567080863364No Hit
GAACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCACTGTG19100.19832431528789735No Hit
GAACTTGCCCATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAAT19050.19780514168766722No Hit
CTCTAGTCCTCGTCCCCGCCGAGTTAAAGTGTCTCCAGAGTAACAACTCT18940.19666295976716103No Hit
ACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCACTGTGGA18290.18991370296416976No Hit
AAATAATGCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAA18120.18814851272338742No Hit
ATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAACCACTATAAGG17820.18503347112200685No Hit
TGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAG17440.18108775176025807No Hit
GAAGAAAATAATGCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTA17340.1800494045597979No Hit
ACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTCAGAAA16870.175169172717635No Hit
GCATATGTCACTGTGGCCCAGGCCTCGCCCATGGGTCCAGAGCCAGTCTC16790.17433849495726683No Hit
CATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCACTACATTT16560.17195029639620837No Hit
GGCGTGGAGAATGCCTATCTTCACCACCATTCTCAAAAGACAACATCAGG15880.16488953543307905No Hit
ATTTCAGAGTCTTCATTTGGAAGAGATTTATTTGATACTATACGGGAATC15830.16437036183284895No Hit
ACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGA15800.1640588576727109No Hit
CTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATAATGAAATG15760.1636435187925268No Hit
CTATTACAGTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAGAT15750.1635396840724808No Hit
GTGCTAATCACGGCAGAGCAAACACCCCATTGTGTAGAAGATGAAATGAG15720.16322817991234273No Hit
GGGCTTAGCTGCTGAAACTGTGGAGCAGGGAGAGAGCAGAAGGTGTTTGG15700.1630205104722507No Hit
GTTAATGCAGCCAGAAGAAAATAATGCAGAGAGCAGAGCTGCTGGTTCTC15680.16281284103215865No Hit
GCACTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGCTAATAATGAA15610.1620859979918365No Hit
CATGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACC15420.16011313831096216No Hit
GACTTGAACCCAGCGAGACCCACTGTGGACATCTGACCTCCATAACTGTA15210.15793260918999574No Hit
GTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGCAGCCAGAA15180.15762110502985768No Hit
AAAGCAGGGCGTGGAGAATGCCTATCTTCACCACCATTCTCAAAAGACAA15080.15658275782939748No Hit
TTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAA15060.15637508838930544No Hit
AGCGAAAGCAGGCAGCCTCTAGAAGGTGAAAATGGCAAAAATCTCCACTA14720.15284470790774077No Hit
GCCGTGATTAGCACTGTAATAGTCAGAAAGCTAATATACCTGGTTGAAGC14720.15284470790774077No Hit
GAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGA14680.1524293690275567No Hit
GTGAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGC14670.15232553430751067No Hit
GGGTAGGGGAGTCAGTCAGGCAGGAATGGCTTTTGCCCCTGACCTGCAGG14540.1509756829469124No Hit
CTAATATACCTGGTTGAAGCTAATAATGAAATGTTCAAGCTCTTGTAGCT14450.15004117046649826No Hit
CTTCTGGCCTCTGGTGTGAACCAGGGAGTGTGTGTGTGTGCACACATAAG14410.14962583158631418No Hit
CTACAAGAGCTTGAACATTTCATTATTAGCTTCAACCAGGTATATTAGCT14330.14879515382594602No Hit
CACATAAGCATATGTCACTGTGGCCCAGGCCTCGCCCATGGGTCCAGAGC14320.1486913191059No Hit
AGAGAGATGAGTTCTGATGATGACAAAGGCTGCTGGCCCATCTCTCCTGG14080.14619928582479552No Hit
GTGTGTGTGTGTGCACACATAAGCATATGTCACTGTGGCCCAGGCCTCGC14020.1455762775045194No Hit
CTGTGGACATCTGACCTCCATAACTGTAAATGTGTAATGTTGTAAGTCAC14000.14536860806442736No Hit
GTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGG13820.143499583103599No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA13540.14059221094231047No Hit
CTATAACGGAAACTAAGGTTCCTAATCAGGGCCTCTTGCTTTGATAGAAG13500.1401768720621264No Hit
GAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGCTGGCCCATCTCT13390.13903469014162018No Hit
GATGATGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCACCTCT13380.13893085542157416No Hit
GGCAGAGGGCTTAGCTGCTGAAACTGTGGAGCAGGGAGAGAGCAGAAGGT13380.13893085542157416No Hit
GTATCTTCTAGTTTCCCAAGTTGGAAGGGTGTTTTTAAGGGTAGGGGAGT13330.13841168182134406No Hit
TGGGTAGTGTCCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAA13310.13820401238125202No Hit
GATGAGTTCTGATGATGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTA13200.1370618304607458No Hit
GTTTTTAAGGGTAGGGGAGTCAGTCAGGCAGGAATGGCTTTTGCCCCTGA13190.13695799574069978No Hit
GTAAATGTGTAATGTTGTAAGTCACTACATTTGTAGTAATCTGTTAATGC12930.13425829301950326No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG12890.13384295413931918No Hit
AATCACGGCAGAGCAAACACCCCATTGTGTAGAAGATGAAATGAGAACCA12880.1337391194192732No Hit
GTCCAGAGCCAGTCTCAGGAAGCGAGAGCCCGACTGGCTCCAGGATGAAC12860.13353144997918115No Hit
ACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCAGAGAGC12850.1334276152591351No Hit
AGCGAAAGCAGGGCGTGGAGAATGCCTATCTTCACCACCATTCTCAAAAG12830.13321994581904306No Hit
GACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGT12780.132700772218813No Hit
GGTATATTAGCTTTCTGACTATTACAGTGCTAATCACGGCAGAGCAAACA12660.13145475557826075No Hit
CTATAGGCTTTTCTGAGAGGTCTGTTGGTTTGTCACACAGCCGCCACAGT12580.1306240778178926No Hit
GTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGTTCTGATGATGA12400.12875505285706426No Hit
ATCTAAATCCCTCTGTTCCCCCTCCCCCCCAGTGATGGAATTTGTTAAAA12350.12823587925683413No Hit
GTGTTTGGGGGCGGTATCTTCTAGTTTCCCAAGTTGGAAGGGTGTTTTTA12320.1279243750966961No Hit
GCAGAGAGCAGAGCTGCTGGTTCTCATTTCATCTTCTACACAATGGGGTG12230.1269898626162819No Hit
GGGAGAGAGAGAGAGATGAGTTCTGATGATGACAAAGGCTGCTGGCCCAT12110.12574384597572968No Hit
GCTGAAACTGTGGAGCAGGGAGAGAGCAGAAGGTGTTTGGGGGCGGTATC11940.12397865573494733No Hit
CCCTAATTCGGTCCTCGCCTAATCAAGGCCTCCCTTAGCCCTCTCTCTAG11920.12377098629485529No Hit
CAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAA11840.12294030853448713No Hit
GGCCTAGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCC11680.12127895301375083No Hit
CTACACAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTCAGA11590.12034444053333666No Hit
CCAATGCAAAGGTCAACAGCAGTGAAAAAGGGAGAGAGAGAGAGATGAGT11580.12024060581329063No Hit
GGAGAGAGCAGAAGGTGTTTGGGGGCGGTATCTTCTAGTTTCCCAAGTTG11560.12003293637319859No Hit
CTTAATGAGGGACCCTAATTCGGTCCTCGCCTAATCAAGGCCTCCCTTAG11540.11982526693310655No Hit
TGACAAAGGCTGCTGGCCCATCTCTCCTGGAACTAGTTCACCTCTTGACC11390.11826774613241627No Hit
TGCCTATCTTCACCACCATTCTCAAAAGACAACATCAGGACCCGTGGCCC11270.11702172949186404No Hit
TTTCATTATTAGCTTCAACCAGGTATATTAGCTTTCTGACTATTACAGTG11200.11629488645154189No Hit
GTCACTACATTTGTAGTAATCTGTTAATGCAGCCAGAAGAAAATAATGCA11130.11556804341121976No Hit
TTTTAAGGGTAGGGGAGTCAGTCAGGCAGGAATGGCTTTTGCCCCTGACC11130.11556804341121976No Hit
GTGTGTGTGTGCACACATAAGCATATGTCACTGTGGCCCAGGCCTCGCCC11090.11515270453103568No Hit
CTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGCCTC11060.11484120037089762No Hit
CAATGGGGTGTTTGCTCTGCCGTGATTAGCACTGTAATAGTCAGAAAGCT10990.1141143573305755No Hit
CCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAA10970.11390668789048346No Hit
CTCCAGAAGGAACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGA10910.11328367957020734No Hit
CACTTAATGAGGGACCCTAATTCGGTCCTCGCCTAATCAAGGCCTCCCTT10860.11276450596997722No Hit
GTTCAAGCTCTTGTAGCTGTGAAGGTCAAGAGGTGAACTAGTTCCAGGAG10820.11234916708979314No Hit
TCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGCCT10790.11203766292965509No Hit
AGTAATAACATTTCCCCTATTTCAGAGTCTTCATTTGGAAGAGATTTATT10730.11141465460937897No Hit
ATACTATACGGGAATCGGCACTTTTCTCCCACACTGTTGGCAAACCACTA10570.10975329908864265No Hit
CTATATCCCTTGGGTCTCACTTCCATCCACCAGCCAGGACACCTGGGCAC10470.10871495188818246No Hit
TTCATGATCCGCTCCATGTTGGCTGTCCAGCCATAGGTGCTAGTCACAAT10420.10819577828795238No Hit
GAAGGAACATAGTCCTGCGGACACCTTGACTTGAACCCAGCGAGACCCAC10420.10819577828795238No Hit
GATAGAGAAAATGACAAGACCAATGCAAAGGTCAACAGCAGTGAAAAAGG10400.10798810884786034No Hit
GGCCCATCTCTCCTGGAACTAGTTCACCTCTTGACCTTCACAGCTACAAG10390.1078842741278143No Hit
CCTTGACTTGAACCCAGCGAGACCCACTGTGGACATCTGACCTCCATAAC10390.1078842741278143No Hit
GATTTATTTGATACTATACGGGAATCGGCACTTTTCTCCCACACTGTTGG10060.10445772836629567No Hit
CCTTCTGGGCCACCGCAGATAGAGAAAATGACAAGACCAATGCAAAGGTC9910.10290020756560536No Hit
CTCTAGAAGGTGAAAATGGCAAAAATCTCCACTAAATCTCCACTAAAGCC9890.10269253812551334No Hit
GAGCAGAAGGTGTTTGGGGGCGGTATCTTCTAGTTTCCCAAGTTGGAAGG9770.1014465214849611No Hit
GAGCAGGGAGAGAGCAGAAGGTGTTTGGGGGCGGTATCTTCTAGTTTCCC9750.10123885204486906No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)