FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995664
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC91290.4574417336786153No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG90480.4533829342013485No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT86210.43198654683353516No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT67960.3405382870062295No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA65060.32600678270490424No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC51190.25650610523615197No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC49780.2494407876275766No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT48660.2438286204491337No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA45770.22934722478333025No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT44220.22158038627744953No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC39410.19747813259145827No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT38560.19321889857210434No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG36950.1851514082530927No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT36470.18274619374804577No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA34090.17082033849385467No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC33760.1691667535216349No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT32620.16345436907214841No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG32400.16235197909066856No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC32320.1619511100064941No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT31980.1602474163987525No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA31950.16009709049218707No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT31380.1572408982674438No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA30450.15258079516391537No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG29090.14576602073294903No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC28430.1424588507885095No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA28100.14080526581628972No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG27070.13564407635754316No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA26880.13469201228262875No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG26770.13414081729188881No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT26370.13213647187101638No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA25700.12877919329105503No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC25100.12577267515974633No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG24990.12522148016900642No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT24770.12411909018752655No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC24690.12371822110335207No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT24400.12226507067321954No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC24350.1220145274956105No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA24130.12091213751413063No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT23650.11850692300908369No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA23120.1158511653264277No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG22910.11479888398046967No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT22850.11449823216733879No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT22830.11439801489629517No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT22740.11394703717659888No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT22650.11349605945690257No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT21830.10938715134411404No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT21470.10758324046532884No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT21270.1065810677548926No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT20770.10407563597880204No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT20580.10312357190388763No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA20290.10167042147375512No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC20260.10152009556718966No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC20200.10121944375405881No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)