FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536201
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC72170.4697952937148199No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG62470.4066525148727283No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA38730.2521154458303308No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA32780.2133835350972952No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA30810.20055969238400445No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA30040.1955473274656116No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT28810.18754056272584121No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC28550.18584807587028002No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT28410.18493673679420855No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA27700.18031494576556062No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG27590.17959889363436166No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC26900.17510729390229535No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC26820.1745865287159688No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA24730.16098153822318823No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA24470.15928905136762703No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT23700.15427668644923417No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT23430.15251910394538215No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT23040.1499803736620403No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA22030.14340571318466788No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT21910.1426245654051781No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT21000.13670086141071383No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT20340.13240454862351997No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA20160.1312328269542853No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC19810.12895447926410672No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA19290.12556950555298427No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC19150.12465816647691284No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA18770.12218453184186183No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT18160.11821369729612205No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT17670.11502401052987207No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC17580.11443814969525472No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA17520.11404757580550982No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA17300.11261547154311187No Hit
ATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGT16740.10897011523882617No Hit
ATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGT16400.10675686319693842No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG16390.1066917675486476No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT16060.10454361115505068No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT15990.10408794161701496No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT15920.10363227207897925No Hit
GCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGT15810.10291621994778027No Hit
AGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC15700.1022001678165813No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA15590.10148411568538232No Hit
GAAATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATT15560.10128882874050986No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA15390.10018220271956599No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)