FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508673
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT79200.5249646543684418No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA62360.4133433818991922No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA58560.38815568383605986No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC57360.38020167392138654No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG50160.3324776144333464No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA45670.30271636066927693No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA41200.2730876737371186No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG38760.2569145202439495No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG36600.24259730239753743No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC35490.23523984322646457No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA33670.2231762615225433No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG32650.21641535309507098No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT30880.20468318847092776No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG28000.1855935646757117No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA27310.18102000897477452No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT26160.17339741613987922No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG25370.16816102627938592No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC23830.15795338022222177No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA23780.15762196314244373No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA23310.15450664259253No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA23150.1534461079372402No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA21450.14217792722478628No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA21310.14124995940140772No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT21290.1411173925694965No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG20290.13448905097393538No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA20070.1330308158229119No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC19840.13150629725593288No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT19210.12733044205072935No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA18940.12554078981992783No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA18210.12070210045516822No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC18140.12023811654347893No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA18100.11997298287965649No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA17530.11619482817018666No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC16610.1100967539022704No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA16590.10996418707035918No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG16390.10863851875124696No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA16330.1082408182555133No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT16200.10737913384809034No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA15820.10486036404177711No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC15430.10227531081950826No Hit
ACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTT15280.1012810595801741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)