FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248211
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG120770.9675447500462663No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA65390.5238697624039526No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT63190.5062445371816143No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA58590.4693917935349071No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA55400.44383521696251677No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT50200.40217559370971734No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC45610.36540296472311173No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT44680.35795230133366873No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC44470.3562698934715365No Hit
TTTTAATACATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCTCC44100.3533056510477796No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT42020.33664180174665986No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA41490.3323957247612783No Hit
AATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGA36510.29249862403071275No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG34470.27615523336999914No Hit
ATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATC32380.25941126940877784No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT30060.2408246682652212No Hit
ATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGT29900.23954283370359658No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA29200.23393480749648896No Hit
GCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGT28990.2322523996343567No Hit
GAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAA28640.22944838653080288No Hit
GCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGA28600.22912792789039674No Hit
AGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTC28050.22472162158481218No Hit
TTTTTTAATACATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCT27340.21903348071760304No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA26810.21478740373222158No Hit
ATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAA26240.21022086810643395No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT26210.20998052412612933No Hit
ACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCAT25790.20661570840186474No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT23340.1869876166769881No Hit
ATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCA23330.18690750201688658No Hit
GATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGG23000.18426371823353585No Hit
TTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATT22380.17929660930724053No Hit
TCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCA22170.17761420144510823No Hit
TCCCTATCCTATGGGGAATTAATGGAAGATCCAGAACTAGAGCCTAGGTT21590.17296755115921908No Hit
AAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAG21580.17288743649911753No Hit
TAGTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGA21530.17248686319860984No Hit
CCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTCAATGACTTCA21130.16928227679454835No Hit
GTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTT20960.16792032757282221No Hit
AGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCA20340.16295321864652693No Hit
GGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTT20270.16239241602581614No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG19780.15846679768084082No Hit
TTCCTATACAGATGGCTCTAATTTGGGAAGTACACTGACTTTTTGACTTG19340.1549417526363732No Hit
ATCCCAGGACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCC18390.14733085992672712No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT18270.1463694840055087No Hit
CCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTGAGCGGCATT18230.14604902536510253No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG18100.14500753478378256No Hit
GGTAAGTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTG18090.144927420123681No Hit
TCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTTAA17920.14356547090195487No Hit
TCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAAT17870.14316489760144718No Hit
CCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGATC17730.14204329236002566No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT17560.14068134313829952No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC17360.1390790499362688No Hit
GGCTAATAGAAGGTAGAAAGTCAATTAGAGACGATTACAAACTAACTGAC16990.1361148075125119No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA16820.1347528582907858No Hit
CATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCTCCATGAATGA16750.134192055670075No Hit
ATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCATTC16670.1335511383892627No Hit
CCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCAT16400.1313880425665212No Hit
GTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTGAGCGG16280.13042666664530275No Hit
CTGTAAGCTTTGAACTACTGACTTGGACATCATTCATGGAGACTTACCTA16230.1300260933447951No Hit
CCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCA16170.12954540538418585No Hit
GAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTTGGCTGT16050.1285840294629674No Hit
CCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGG15710.12586013101951513No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT15700.1257800163594136No Hit
GGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATC15610.12505898441849975No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG15460.1238572645169767No Hit
CTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAA15180.12161405403413364No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA15000.12017199015230598No Hit
GAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTT14800.11856969695027524No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA14770.11832935296997062No Hit
TATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAAT14580.11680717442804141No Hit
ACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCC13810.11063834560022304No Hit
AGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCC13730.10999742831941074No Hit
GGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAAT13640.1092763963784969No Hit
CTTCAGATAGGTAAGTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAG13620.10911616705829383No Hit
TCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTCAAAAGCTT13290.10647238327494309No Hit
GGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGTGGGACT13210.1058314659941308No Hit
TTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCA13190.10567123667392772No Hit
ATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAG13130.10519054871331851No Hit
CTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATG13120.10511043405321698No Hit
GTTCTGCATTTTTAAAATTAAATTCCTTAGAGACAAGCTAATTTGCCAGA13100.10495020473301389No Hit
GAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTCAAAAG13080.10478997541281082No Hit
GGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAGAGACAA13070.10470986075270929No Hit
AGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCT12940.1036683701713893No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA12790.10246665026986622No Hit
ATACTGTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAA12570.10070412774763242No Hit
GCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGTTTTGT12500.10014332512692165No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)