FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456624
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG60670.4165110557014027No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC57950.3978377398697262No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA33910.2327985808279968No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA27700.1901657531387647No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA27600.18947923417436482No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA26420.18137831039444632No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA24280.16668680455628906No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT22970.15769340612265073No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT22660.15556519733301113No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC22370.1535742923362515No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA22350.1534369885433715No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT22240.15268181768253167No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT22160.15213260251101177No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC22040.15130877975373191No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT21940.15062226078933205No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT21390.14684640648513275No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT21090.14478684959193314No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA20620.14156021045925374No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC19920.13675457770845462No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA19490.13380254616153517No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT19280.13236085633629543No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG18520.1271433122068564No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG18410.12638814134601656No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT17580.1206900339414976No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG17580.1206900339414976No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC17450.11979755928777777No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG17020.11684552774085832No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG16910.11609035688001845No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC16890.11595305308713848No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA16390.11252045826513912No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT15880.10901921154669977No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA15830.10867595206449983No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA15660.10750886982502003No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC15590.10702830654994012No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA15100.10366436362438075No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA14810.10167345862762112No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA14780.10146750293830117No Hit
ATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGT14600.10023176880238138No Hit
GAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAA14570.10002581311306144No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)