FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585265
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT78310.49398680977628345No Hit
ACATAAGGGTGCATAGATCTTTCTAAACTTATGTTTTTGTTTTGTTTAAT34680.21876468603041133No Hit
CCTCTAGGGTGCGCCTGTCCCTGAGTGCCAAGACTTATGAGCTAGATGGC28430.17933910103358114No Hit
GTTCTTAGGTGTGACAGAAGAAAATTAATGAACGTGCCCTTCCACATTTG26930.1698769606343419No Hit
CCTATGGATGGACCCTTGCATTACTTCCATATCTTGGCTATTGTAACTAA26530.16735372319454475No Hit
AAACATAAGGGTGCATAGATCTTTCTAAACTTATGTTTTTGTTTTGTTTA25560.16123487240303672No Hit
TTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAAGGCAAAGGAT25460.16060406304308744No Hit
TCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCTCTGGCTCCACTC24570.15498985973953883No Hit
ATTTATGAGTCTGTCTGCTTTGTCTATTTTTTCTTAGATTCTTCATGAAA23900.15076343702787862No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA23490.14817711865208655No Hit
GCATTAAACAAAGCAAACAAGGCAAAGGATTTGAATAGATATTTCTCCAA23000.14508615278833506No Hit
GAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAAGGCAA22810.14388761500443145No Hit
TCTATGTGTTGAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCA21440.13524552677312626No Hit
ATTGTAACTAATGCTGCAGTAAACATAAGGGTGCATAGATCTTTCTAAAC21410.1350562839651415No Hit
GTCTGGGGCGGTCTGTGAGCAGGATCAGCTGGGCATGGAATGGGGCCCAC20200.12742349070975514No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG19910.12559414356590223No Hit
CATTTATTGAGAATCTATGTGTTGAATATTGTAGGTCCAAGTGATAGAGC19060.12023226400633333No Hit
GTTGAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAAGG18630.11751978375855142No Hit
AACTATACCCATCCATGAAATCATTCAGCTTCAGCATTATAGCAGATGTT18270.11524887006273399No Hit
GTGTTGAATATTGTAGGTCCAAGTGATAGAGCATTAAACAAAGCAAACAA17630.11121169015905859No Hit
TCTTTATCCATTCACCTATGGATGGACCCTTGCATTACTTCCATATCTTG16970.1070483483833933No Hit
GACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGT16540.1043358681356114No Hit
CCCCTAGGCTGGAGTTGCCATCAGTGAGAAGGGGAAGTACATGGGGGTTA16500.10408354439163169No Hit
GTTCTGCTCCTTCCCTCTGTGGGCCCCATTCCATGCCCAGCTGATCCTGC16470.10389430158364689No Hit
GTCTACCTCACCCTGAATGGTAACAGACTGAACGTTTTTCCTACAAGATC16430.10364197783966718No Hit
GTACAAACTACTACTAACAATGCAAGTCATACGGTGCCTGGGTAGCTCAG15930.10048793103992076No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)