FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_A08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_A08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729998
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT40140.23202338962241575No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA29370.16976898239188715No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA27320.15791925771012452No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG26450.15289035016225452No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA20830.120404763473715No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC18640.10774578930149052No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA18610.10757237869639155No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG17340.10023132974720202No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)