FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536057
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC70650.4599438692704763No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA51390.33455789726553115No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT49920.3249879399006677No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT47260.3076708741928197No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA45090.2935437942732594No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG41340.2691306377302405No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC38140.24829807748019767No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA36910.2402905621340875No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG34120.2221271736660814No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG33900.22069493514889096No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA33680.2192626966317005No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT32890.2141196583199712No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG32420.21105987603324614No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA31260.20350807294260564No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG30640.19947176439415984No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT28070.18274061444334422No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC28010.1823500039386559No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA26460.17225923256754144No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC26150.17024107829331855No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT25870.16841822927143982No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA25360.16509803998158923No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA24980.16262417345189664No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA24200.1575462368909487No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC22580.14699975326436454No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC22220.14465609023623474No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA22090.14380976747607674No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG21660.14101039219247724No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC21590.14055467993700754No Hit
GAAATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATT21230.13821101690887772No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA21050.13703918539481283No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC20610.13417470836043194No Hit
ATATAGTATCTGGCCCAGGCATACTATAATTTGACTGTTAGCAGATTCTT20570.1339143013573064No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA20490.13339348735105533No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT20230.13170084183073935No Hit
CATAAAAAGTTATTCTATTATTCATGCTTGTATTCTGGACTGAGATTAGT20170.13131023132605105No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC19610.12766453328229355No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT19210.12506046325103823No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC18530.1206335441979041No Hit
ATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGT18110.117899270665086No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG17950.11685764265258385No Hit
CTATAATTTGACTGTTAGCAGATTCTTTTAGCTACCAAGAGGACACAGTA17760.11562070938773758No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG17640.11483948837836096No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA17570.11438377612289127No Hit
GGAAAAAACTACTCTCAAGTTATCAAAGGTTCTTCTGTTAAAAGTGTGAT17370.11308174110726359No Hit
GTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGTTCTTCTGT17010.11073807807913379No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA16590.10800380454631567No Hit
ATTCTGGACTGAGATTAGTCTAGAGCTGAGAACAGGATTGGGTATTCTAA16540.10767829579240873No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG16400.10676687128146937No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT16310.10618095552443693No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA16150.10513932751193478No Hit
CAGTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAA16140.1050742257611534No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG16120.10494402225959064No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC15750.10253525748067943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)