FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276071
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT75060.5882117844539998No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA65620.5142347095106777No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG63710.4992668903219335No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG53680.4206662481946537No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG50340.39449215600072407No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA45990.36040314371222293No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT45740.3584440050749527No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC40230.3152645895095179No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA38550.30209917786706225No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA34230.2682452622150335No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT29950.23470480874496794No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG28430.22279324583036525No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA27550.21589707782717418No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC26300.20610138464082325No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC24920.19528693936309188No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT23990.18799894363244676No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT22490.17624411180882568No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT22320.17491189753548195No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA21590.16919121271465304No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG20740.16253014134793442No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT19730.15461522125336286No Hit
ATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATA19500.15281281370707428No Hit
GATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCC19430.15226425488863865No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA19260.15093204061529492No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT18560.1454464524309384No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT18340.14372241043014064No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA18130.1420767339748337No Hit
AACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAA18120.14199836842934288No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA18100.14184163733836128No Hit
GACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGA18020.14121471297443483No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG17890.14019596088305433No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC17880.14011759533756352No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT17600.1379233600638209No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT17160.13447527606222537No Hit
ACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAAC16970.13298633069790003No Hit
TTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATG16960.1329079651524092No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA16070.12593343160372739No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA16050.12577670051274575No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA16040.12569833496725497No Hit
ATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGT15940.12491467951234689No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA15730.12326900305703993No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA15230.11935072578249956No Hit
CCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAA14890.11668629723581211No Hit
ACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAA14880.11660793169032131No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT14730.1154324485079592No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG14620.11457042750756032No Hit
CTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGA14560.11410023423461547No Hit
TCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAA13850.10853628050476816No Hit
CAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACA13580.10642041077651636No Hit
TCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGAC13460.10548002423062666No Hit
CTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCA13200.10344252004786568No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC13190.10336415450237486No Hit
TTTTTATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGA12840.1006213604101966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)