FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n01_S10_R1_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n01_S10_R1_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240685
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT36680.2956431326243164No Hit
GTCTAATATTAACATGTAACTGATTTTGCCGTTTAGGCTTTAGCTTTTAA20590.16595670939843715No Hit
CATTAATACTGAGACTTAGAAAATTAAAGGCAGCTTAAGGTTGCCAAAAT20180.1626520833249374No Hit
GTATTAATTCATGTGATTTATTCTAAGGAAATATTAGAAATGCTCACAAT17470.14080931098546368No Hit
CAACTACACCGTGTGTGTGAACCGCCAGCCACTTCTCCAGCTGAAGGAGG15870.12791320923522087No Hit
TTCTAGGACTTACCACTACTTTAACACTTAGGGTAAATGCATACATTTTA15710.12662359906019657No Hit
GTATTAAGAATATGAAAGTACCCATGTCTTCTAGGACTTACCACTACTTT14820.11945014246162403No Hit
GTACAGAAAAGTATTAAGAATATGAAAGTACCCATGTCTTCTAGGACTTA14760.11896653864598991No Hit
GAATATGAAAGTACCCATGTCTTCTAGGACTTACCACTACTTTAACACTT12600.10155680128316213No Hit
TATTAGAAATGCTCACAATGGTTTAGAAGTTAGGTTAACAGTGAAATGCA12440.10026719110813784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)