FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9469791
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG460660.4864521297249327No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN264460.27926698699052593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG42600.014.03432145
GCCGACC58100.013.497567145
CTATACT82700.011.7476174
TCTCGGG55650.011.58039145
TCTATAC74350.011.4027543
ATACCGT37600.011.2723096
TATACCG42750.011.1344115
GTATATA266500.011.0433581
TATACTG95450.010.6568585
GTATAGG57950.010.5851311
GCTGCCC54550.010.390546145
TATACGG48250.010.2706235
ACTGTGC32400.010.2625438
GTATAGA103700.010.068461
ATACAGG59700.010.0484926
GTATAAG93850.010.0126731
TTATACT176200.09.8063854
TCGCCGG63350.09.805137145
TATACAG123100.09.6933425
GTATTAG95900.09.6625451
Produced by FastQC (version 0.11.5)