FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_s.061.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_s.061.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770027
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG5550.047.57706145
TCTCGGG8700.025.887518145
CTACTCG8750.024.852016145
CCTGTCG4907.2759576E-1222.1893145
GCTGCCC10550.022.084137145
GCCGACC14850.021.965166145
TCGCCGG15650.019.849854145
CGGTGGG12900.019.26513145
CTATACT16300.018.3939194
ATCTACG12400.017.536707145
TAGATCG9800.015.849501145
CTGTGCG3306.259264E-515.8008379
CCGACGC15250.015.787142145
ACTATAC17350.015.7780683
CCGACGG4058.0546066E-515.340751145
TCGCGGG5653.83905E-715.120143145
TATACGG14400.014.9367275
ATACGGA14550.014.7827426
AGGGGTC4453.026271E-714.6468439
ACTGTGC5851.789158E-814.4840998
Produced by FastQC (version 0.11.5)