FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_s.060.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_s.060.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415258
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1950.051.850876145
TATACGG3000.030.3556165
ATACGGT2502.4738256E-1028.6210086
GTGTAGG1955.0594645E-627.919703145
TACGGTG2603.9108272E-1027.52027
CGTCACC950.00802491227.3885258
CAGCTCG950.00802962927.3852279
GAGGACG1207.6523033E-427.0999669
GCCGACC5450.024.260502145
ACGATCG3251.8852006E-823.931173145
CGCCGTG1951.6217117E-423.931173145
ACGGTGT2802.5862391E-823.2313398
CGGTGGG4703.6379788E-1223.167414145
GTATAGA3005.3507392E-821.6904181
GACTGTA1904.5589256E-420.5413937
ATAGGCT1955.4315437E-420.014698
GCTATAC1650.004868198719.7138143
TCTAAGC1650.004868198719.7138143
GGATAGA1650.004871591519.711446
TATAGGC2006.44147E-419.5143247
Produced by FastQC (version 0.11.5)