FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086313
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG4500.044.988445145
TCTCGGG8850.036.072872145
CTACTCG7150.025.04736145
GCTGCCC7000.023.359386145
CCGACGG2904.44694E-621.479895145
AGTCTGC2006.522821E-419.4821058
CGACGAG5807.4578566E-1118.794909145
GTCTGCC2451.19199336E-418.5543849
TCTGACC5951.07320375E-1018.321087145
CTGCACT3603.744899E-718.0398164
TACGGTG4056.646769E-817.6381197
CCGACGC12350.016.392551145
ACGATCG7651.2732926E-1116.28541145
TCGCCGG11000.016.280783145
TATACCG4802.5966074E-816.2358345
TGCACTG3658.511901E-616.0134265
ATCTACG7801.8189894E-1115.97223145
CTGTCTG7401.4188117E-1015.783368145
ACTCTGG6959.822543E-1015.684959145
CGACGAA14900.015.67744145
Produced by FastQC (version 0.11.5)