FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_C4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_C4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566521
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG2350.049.62684145
GCTGCCC4750.029.462667145
ACAGTGC900.006152815728.9218778
TACAGGC1457.284617E-526.9272677
TCTCGGG5850.026.580753145
TATACGG2456.2354957E-926.5609075
ACCGTAC1551.1463983E-425.1900238
TAGATCG3751.2732926E-1024.879585145
ATACAGG3352.8921932E-1023.3101716
GGACTGG1400.001874725523.2407956
CCGTCTA1400.001874725523.2407959
TCTGACC3753.4542609E-922.806288145
GCCTTAG2002.4521454E-522.7820131
TAGTCCG6000.022.775989
AGGGGTC1500.002799127521.6914089
ACTCTGG3605.6053977E-821.596863145
GTACCTC1550.003385467520.9916866
ATAGCGA2205.125825E-520.7054378
TACGGTG2256.0938248E-520.2453167
CCTGTCG2355.742727E-419.850735145
Produced by FastQC (version 0.11.5)