FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_C3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_C3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530633
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG2250.041.55389145
CAGTGCG1153.3733886E-739.4930579
CGGTGGG5900.027.73194145
ATGGCGA1457.4323165E-526.84758
CTACTCG3805.456968E-1226.654634145
GCTGCCC4450.026.262993145
TCTATAC3204.472895E-922.3033
TCGCCGG9900.022.036156145
GTATAGG2954.6118657E-821.9999331
TCAGTGC1550.003443906820.92958
TCTGACC4151.0842996E-820.651783145
ATCTACG5402.7284841E-1120.199808145
TACTAGG2006.544252E-419.469942
TCTCGGG4502.7490387E-819.045534145
CGACGAA9000.018.179829145
GTACTAG1800.00816056218.0277231
ACAGTGC1800.00817387518.0226278
ACGATCG3902.8179675E-617.98005145
TAGTACA3252.8394861E-617.9671734
TATACGG2952.4848548E-517.594975
Produced by FastQC (version 0.11.5)