FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773658
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG8050.035.288242145
CGCCGTG5500.025.126614145
TCGCCGG18900.023.96704145
GCCGACC10400.022.885084145
GCTGCCC10250.019.474829145
CCGACGC9350.018.886017145
TCTATAC12800.017.6015153
TCTCGGG11100.017.29184145
CGGTGGG15550.017.28072145
TAGATCG9850.017.147886145
TATACCG9100.016.7472325
GGATAGG7951.8189894E-1216.41746145
ACGGTGT7900.015.9356918
CTATACT16900.015.6830274
TACGGTG8900.015.6341287
GCCGTAG5904.197682E-815.61541145
TCTGACC10500.015.355153145
ATACGGT10800.015.3381466
GTCGCCC8607.2759576E-1215.176605145
GTATATG20100.015.1697211
Produced by FastQC (version 0.11.5)