FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399939
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG5950.024.495682145
TCTCGGG6900.024.458313145
TCGCCGG11500.024.013617145
GCTGCCC6400.022.773329145
ATACGGT8800.022.6195186
TGACGCG5850.022.291842145
GGATAGG5101.4551915E-1121.057692145
CGGTGGG10950.020.315933145
TACGGTG7650.019.9464077
TATACGG9700.017.7846955
GCCGACC5651.3042154E-917.650126145
CTATACT14300.016.7036444
GCCTTAG4801.9917934E-816.590021
CTGCCGC25550.016.51295145
ACGGTGT7650.016.4774678
TTAGACT8300.015.9880934
CTTAGAC6251.2369128E-1015.925283
GTATAGA11500.015.5801931
TATACCG6852.910383E-1115.4979485
ATACACG4301.9183135E-615.430374
Produced by FastQC (version 0.11.5)