FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408771
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG7100.031.504303145
CCGACGC7350.024.136301145
GCCGACC7750.023.8858145
CTAGGAC1951.8518302E-523.6286353
GCTGCCC6350.021.863968145
TCTCGGG7100.021.727106145
CGGTGGG9950.021.70527145
CTAGGGT2409.1800954E-519.1975844
TCGCCGG12400.018.66078145
ACTGTGC3954.287358E-818.3283988
CCTATAC7550.018.3082773
TATACCG7550.018.3076295
AGGGGCC2650.001359922217.463675145
TATACGG8650.016.7404215
TAGATCG7201.07320375E-1016.069006145
CAGGGCC3100.003875059514.928624145
CTATACT13350.014.7910754
TACAGGG4503.344543E-614.6267317
TAGGACA6352.9558578E-914.51155954
GTATTAG12250.014.5109351
Produced by FastQC (version 0.11.5)