FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477208
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG9700.027.744795145
GCCGACC12350.026.772364145
CGGTGGG14150.023.910105145
TCTCGGG13150.023.38942145
GCTGCCC12500.023.375387145
TACGGTG11450.022.518747
CCGACGC10350.022.287745145
TAGATCG9600.021.626078145
ACGGTGT12050.020.2985298
TCGCCGG19300.019.920393145
TATACGG16950.019.1126165
ATACGGT14650.018.5029076
GCCTTAG7900.017.5799431
GTCGCCC8750.017.575478145
TCTGACC16650.017.08727145
GTATATA84300.016.1608751
ATACAGG19850.015.6542076
CCTGTCG7451.891749E-1015.481755145
TATACCG14200.015.3645325
TAGGACT9950.015.2826444
Produced by FastQC (version 0.11.5)