FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585667
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC450.0076454351.248444145
CGGTGGG5250.035.141792145
TAGATCG3250.033.11438145
TGACGCG2708.731149E-1131.318497145
CGTACCG850.00429925831.1221879
CCGACGC3750.030.749067145
GATGGAC1752.2572385E-730.2329838
TCTCGGG3850.029.95039145
GCTGCCC2652.4338078E-929.008554145
GCCGACC3252.3646862E-1128.383755145
TCGCCGG6450.025.028309145
TCTGACC2901.8637911E-723.857035145
ACTGTGC1400.001706528223.6195187
GTCGCCC2652.2696622E-623.206842145
ATGACCG1500.002548527222.0448825
CTATACT5150.021.8308544
CTGTGCG1550.003082695421.3337578
TAGGACA1954.8607297E-420.3491214
TCTATAC4252.0918378E-1020.231153
GTCCTAC1650.004421798520.0459381
Produced by FastQC (version 0.11.5)