FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304908
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG5950.030.963654145
TCTCGGG8100.028.431133145
GCCGACC5650.023.09715145
CCGACGC7750.021.791088145
GCTGCCC6450.021.422527145
TAGATCG7350.019.843771145
CGGTGGG10450.019.833776145
TAGGACG2159.25199E-418.4957524
ATACAGG10550.017.5899566
TAGACTG4207.9833626E-817.3581165
TATACAG16800.016.9636145
CCTAGCG2350.001680993916.9196999
GTATAAG11000.016.8748951
TACACCG5203.0213414E-916.569117
TAGGACT4501.7525599E-716.2009094
GTATTAG11750.015.7977731
TATATAC45350.015.638043
TACAGGC5951.0695658E-915.5944577
AGGACTC3457.930379E-515.3684515
ACAGTGT5205.4222255E-815.2928068
Produced by FastQC (version 0.11.5)