FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641427
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC2506.35373E-926.516033
TCTCGGG3608.913048E-1125.587952145
TGACGCG2751.13117494E-725.122717145
CGCCGTG2251.7026416E-523.88209145
ACGGTGT2802.120396E-823.6750268
TACGGTG2805.544516E-721.3075247
CGGTGGG6600.020.935598145
CTACGTA3251.028493E-720.3937669
CTAAGGG1650.004369051720.0879024
AGTCTGC1650.004369051720.0879028
GCCGTAG2356.259406E-419.599283145
GTCCTAA2408.6828084E-519.3346061
ACTACGT3451.92671E-719.2145148
GCTGCCC3351.7463943E-518.331669145
GCTGCTC2551.3822895E-418.1972758
CTATACT6600.018.0791114
TAGATCG3001.5598728E-417.911566145
CCGACGC3903.2505377E-617.714737145
CCTACCC2702.1429622E-417.1836369
GCCGACC4054.657817E-617.058634145
Produced by FastQC (version 0.11.5)