FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142564
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG4300.039.346344145
TCTCGGG5850.027.60664145
GCCGACC6000.026.916473145
TAGATCG6450.026.230894145
CGGTGGG9000.022.216772145
GTAGACG2754.800022E-721.6324624
AGACGGC1904.0974593E-420.8734266
TCTGACC6501.8189894E-1218.930267145
GCTGCCC5851.0186341E-1018.404427145
GTACTAG2901.8263028E-518.2398451
TCGCCGG10550.018.223745145
CACATCG5106.8030204E-918.095108145
CCGACGC6853.6379788E-1217.963028145
ACTGAGC3555.5331748E-616.7560718
TATACCG5152.752131E-916.685235
TAGACGG2400.001968405416.5247945
TACGGTG5354.5620254E-916.0614837
CTGCCGC26450.015.991423145
GAGTCCG3759.317369E-615.8617219
TATAGCG3509.1715636E-515.1083835
Produced by FastQC (version 0.11.5)