FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772969
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG1351.1574157E-633.742889
TGACGCG4150.033.72057145
GCCGACC5250.023.693605145
TACTCCG2201.916842E-623.665375
AGTGCTC1952.0202511E-523.3619698
TCTGACC5100.022.866074145
ACAGTGC2205.1255636E-520.70728
CGGTGGG9800.019.832817145
CTAGAAG6350.019.4752083
GTGTAGG4001.7076127E-719.436161145
ACTGTGC1750.006829688318.5942218
GCTGCCC3852.547149E-618.174074145
TATAGTG5502.3646862E-1117.749035
CTCCGCA2200.001219909217.747889
ATACCGT2952.4228535E-517.648756
GCCTCAG1850.00940836417.5891277
ACCGATG2652.1367848E-417.1908828
CGCCGTG2750.001651617216.96247145
ACGGTGT3908.464249E-716.687128
CCCTTGA2350.001894001816.6193371
Produced by FastQC (version 0.11.5)