FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545259
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG6700.033.19425145
GAGTCCG5055.456968E-1219.7119949
GCCGACC8200.019.640163145
CTAAGGG3552.5717054E-718.698243
GAGAGGG12850.017.904316145
GCTGCCC8150.017.878687145
GTAGGCC3353.0632636E-617.8296344
TCTCGGG13850.017.165304145
TCTATAC12000.016.5946883
CTTACAC6108.54925E-1116.3226433
TAGGCCG2450.002199657316.2528655
ACCGATC3305.3631815E-516.0886948
ACTGTGC4402.4311194E-615.0831518
CCGACGC7652.928573E-1015.037286145
AGAGTCC5357.5517164E-814.8858018
CAGTGCG2700.004203392714.7474929
TATACAG18550.014.6684655
CTATACT16300.014.6574914
TCAGTGC5459.474388E-814.6126678
AGAGTGT7801.4551915E-1114.4643546
Produced by FastQC (version 0.11.5)