FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077783
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC4900.033.058224145
GCCTTAG3100.031.8529931
TGACGCG4500.030.854343145
GCCGACC5150.029.955671145
GCTGCCC5250.024.977327145
GTCCTAG1752.4159517E-422.5701221
GAGAGGG8200.020.694986145
AGACTCC4252.2555469E-1020.1304826
TACGGTG4754.7293724E-1119.3969847
CCTTAGA6150.019.2644942
ACGGTGT5051.1459633E-1018.2438418
TAGACTC4708.6401997E-1018.2030945
TCTGTCG3903.1085292E-617.800583145
CTTAGAC5552.3646862E-1117.788463
TCTGACC6552.5465852E-1117.6647145
TCTATAC7300.017.1305313
GTGCCTA2702.2648947E-417.0613699
TTAGACT7000.016.9228764
CTGTGCG2350.001761040116.8020789
AACTGTG6701.8189894E-1216.6983627
Produced by FastQC (version 0.11.5)