FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295251
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG14550.026.325651145
GCCGACC16450.025.613495145
GCTGCCC16700.022.477694145
CCGACGC14500.020.076487145
GAGAGGG25400.017.79469145
TCTATAC26650.016.9629293
CCTGTCG13050.014.675794145
CTGCCGC73150.014.557023145
CTAGGAC6905.347829E-1014.4521123
GTATAGA30400.014.4363831
TATACGG21200.014.1104045
CGGTGGG32650.014.077974145
ACTATGC24200.014.0080618
CACATCG21950.013.960391145
TACAGGC13450.013.8377537
TATACTG35400.013.5204775
GTATATA103350.013.3182731
ATACAGG26150.013.2188736
TATACCG17150.013.1788535
TACGGTG17250.013.1014597
Produced by FastQC (version 0.11.5)