FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693304
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG3100.032.178513145
ACGGACC1000.00938255926.5271116
GCTGCCC4050.026.525116145
GTCTAAC2101.0799668E-625.2766781
GCCGACC4202.3646862E-1123.750807145
TCTGACC4805.456968E-1222.380566145
AGGGGTC2204.4345976E-521.0995889
CACATCG5101.4551915E-1121.064062145
ATACCGT3208.6820364E-820.7243066
TAGGGAG2306.241063E-520.1836724
CTGGCCA2306.244528E-520.1822159
GCTATCC2005.649179E-419.9010733
TATACCG2709.602865E-619.6497125
ACCGTCT2056.684141E-419.4100828
TAGGGCA1750.00612763518.946579
CCAGGAC1800.007194555418.4269223
TAAGCAG3252.3056818E-618.3649235
GGCCATA2200.001074423918.0866667
TACTATG7400.017.9237237
TAATGCC3805.293259E-717.4520474
Produced by FastQC (version 0.11.5)