FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853087
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG3650.035.75328145
GCCGACC4300.035.704372145
TCTCGGG8750.021.932684145
ACAGCGC2204.4604145E-521.084658
GCTGCCC4801.4551915E-1020.790358145
TCTGACC4901.9281288E-1020.366064145
CGGTGGG12500.019.651686145
TGCAGTC2709.672074E-619.6343847
TACAGGC3451.9359322E-719.2075527
CTGCTCA2901.791854E-518.2792179
AGTCCAC2200.00108034318.0725578
GCCTAAC1900.00985693817.447641
ACTGAGG3052.7630833E-517.3812588
TCGCCGG9400.017.149492145
GTCTTAG3103.1626307E-517.1099411
GCCTTAG2802.823073E-416.5752561
TAGTCCG6451.0913936E-1116.4371269
CCTAAGA2954.2065763E-415.729682
GACTGAG2954.2178496E-415.7241487
TGCCGCG2950.00287366215.613098145
Produced by FastQC (version 0.11.5)