FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805431
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG4800.043.170357145
TCTGACC5050.022.796228145
GCCGACC5050.022.796228145
CCTGTCG3754.0199666E-922.512543145
TAGACCA4155.456968E-1222.3669825
GACCGCT1500.002513090422.0993357
GCCTTAG3106.21003E-821.3930951
GCTGCCC5401.8189894E-1221.318697145
CTAGACC3251.0256372E-720.4006524
GAGAGGG7850.019.553452145
GGAGTGC2107.874544E-418.9434646
CTAGGGG2050.006570728518.718853145
ACTTCGC3201.9933668E-618.6474728
TACAGGC3953.9372026E-818.4627367
ACTAGAC4004.5438355E-818.2330863
ATACGGT4106.0268576E-817.7883746
ACACCGA2300.001450044717.2962066
TGGACTC2350.001674600516.9282025
GTCTCAC2400.001924614816.5796491
TAGACCC2853.230196E-416.2847334
Produced by FastQC (version 0.11.5)