FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947918
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5750.029.392382145
TGACGCG4850.025.343008145
AGGGTCG2002.1551265E-523.168488
GCCGACC5251.8189894E-1221.948854145
TAGACTC3957.8216544E-1121.787065
GGGTCGA1904.0566197E-420.903899
ACTGGGC1954.8336363E-420.3678918
GTCCTGA3401.6737067E-719.4754751
TACGGTG4203.929017E-918.9140427
GAGCTCG2851.047672E-418.868315145
CCTGTCG2458.2482933E-418.813307145
CCGACGC4954.7584763E-918.623272145
TCTGACC6051.6552804E-1017.776758145
ATACGGT5251.8371793E-1017.6531056
GAGTCCG3002.4197554E-517.6521749
ACGGTGT4157.054405E-817.5458358
GTACTAG3454.139645E-617.27391
GCCTTAG3606.2071904E-616.5541521
ACAGTCC2400.001948978616.5489148
ACAATGC6501.2732926E-1116.2943158
Produced by FastQC (version 0.11.5)