FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727853
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG9850.031.319088145
CGCCGTG4950.028.04482145
TCTCGGG10850.027.01091145
GCTGCCC10450.024.354712145
TACGCGG11500.020.789747145
GCCGACC12300.020.691605145
TAGATCG9850.019.57443145
AGACTCC5400.019.505326
CTATACT18500.019.2153764
TATACCG7600.019.0561835
CTAGCCT4254.8839865E-918.5874214
TCTATAC14100.017.7435933
CTAGGCT3002.5395258E-517.5547854
ACTTCGC6007.4578566E-1116.456668
CTGTGCA5604.984031E-1016.4561829
TACTCGG2903.8994805E-415.8887287
GCCTTAG5405.4278644E-915.8535781
TTACTCC17600.015.7095384
TAGGACT6351.7644197E-1015.55054
CTGTGCT8950.015.444919
Produced by FastQC (version 0.11.5)