FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684595
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG11050.030.683788145
TGACGCG10050.026.836172145
TACGCGG15350.022.590338145
GCTGCCC11250.021.233803145
TCTCGGG11500.018.761984145
TCGCCGG18800.018.44477145
CCGACGG3401.9231047E-518.131329145
ATACAGG12250.017.7550776
GCCGACC14000.017.61329145
GAGTCCG4901.4788384E-917.4845059
GTATATA54000.017.2162171
TAGTCCG14150.016.7668749
CGGTGGG18200.016.089066145
TATACCG9150.015.8469725
CTTAGCC3801.0828153E-515.6132713
TTACGCC8003.274181E-1115.41163145
CGCGTGG10050.014.568208145
CTATACT17650.014.5634624
AATGCGC5056.9093585E-714.3551389
ATAGTCC17550.014.2704718
Produced by FastQC (version 0.11.5)