FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319263
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG1508.829498E-526.1804499
TCTCGGG8050.025.971756145
TACGCGG10450.023.71194145
TCTGACC4950.023.464941145
ACACCGA5250.019.9492786
TGACGCG8600.019.808777145
GCCGACC10250.019.641872145
CGGTGGG12350.018.809952145
TACACCG4306.0535967E-918.2675065
GCTGCCC6953.6379788E-1217.826603145
GCCTTAG4056.0594175E-817.782241
CCATGTG3704.6020068E-717.6894939
TCTAGGG2652.0448859E-417.29162
CCGACTC2350.00182658616.7109269
GAGTCCG4351.3741737E-716.550869
CTGTCCA5554.765752E-1016.5101959
GGACTGC2400.002101002316.3646416
CTGTGCG2803.1165E-416.3627829
ACAGTGC4451.7783532E-716.1801578
GCGTGTT8650.016.113497145
Produced by FastQC (version 0.11.5)