FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057656
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG15450.029.890942145
TACGCGG21900.025.304934145
GCCGACC23500.024.237103145
TCTCGGG18600.022.759703145
CGGTGGG26100.022.11758145
GCTGCCC17300.021.800518145
CGCGTGG14300.017.762117145
TATACAG40200.017.7357445
ATACAGG28100.017.3850986
TCGCCGG30350.016.991549145
AGACTCC10450.015.7933926
TAGATCG15600.015.788548145
CCGACGG6901.0495569E-915.616934145
TACAGGC15250.015.1505567
GTATATA101500.014.7045041
CCTACCC21300.014.2558939
ACGGTGT15350.014.1917518
GCCTTAG10300.014.1056611
CTATACT30950.014.0778074
TACGGTG14550.014.0646557
Produced by FastQC (version 0.11.5)