FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613094
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG5100.036.31696145
GCCGACC6650.029.01261145
TGTACCG950.00761173527.6889155
TCTGACC4450.026.013554145
CTCTACG1350.001423186724.3679181
GTCCACA1901.5179221E-524.2396661
AGACCGC1651.6320024E-423.9131556
TCTATAC4450.023.6502533
ACTGTCC2052.7423845E-522.4550368
GCTGCCC4154.984031E-1022.31524145
TACGGTG2755.034872E-721.5218377
ACGGTGT2755.034872E-721.5218378
GCGTGTG1800.002997061521.437096145
CGACGAG4059.147698E-920.960716145
TCTCGGG5551.8189894E-1220.857716145
CGGTGGG8150.020.832123145
ACAGTGC1955.0502917E-420.2342076
TAGATCG4652.062734E-919.915752145
TATACGG4002.3228495E-919.7283525
AGTCCCC3351.5376463E-719.6302018
Produced by FastQC (version 0.11.5)