FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965375
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA10750.111355690793733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG6100.026.535992145
CTACCCC3855.820766E-1122.2428264
TACGCGG9150.021.06031145
GCTGCCC6350.020.635788145
CGCCGTG3851.2505188E-720.020971145
TATACCG5600.019.9971565
ACCCCTT4302.6011548E-1019.9150896
CGGTGGG9400.019.680187145
CCCTTGC4052.6593625E-919.5179218
TCAGCGC3051.3402478E-619.437938
ATCAGCG4204.1763997E-918.8208547
TATACAG13800.018.616285
GTATATA33200.018.2596341
TCTCGGG11050.018.136644145
GCCGACC7750.017.902622145
CCTTGCG4055.6450517E-817.8914289
GGTGTGC1850.00877585317.803518
GAGCGCA2601.68434E-417.7350359
ACTGCTC3353.2864828E-617.697228
GACAGGT3002.523599E-517.566137
Produced by FastQC (version 0.11.5)