FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874886
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG10800.030.712898145
GCTGCCC6800.026.091257145
TCTCGGG8900.026.002037145
CGCCGAG2851.5256956E-724.3598145
CGGTGGG12300.021.950228145
GCGTGTG3204.6630885E-721.69545145
AACCGCT2153.938908E-521.4276087
GTGTAGG4007.9835445E-921.213327145
TCTGACC5551.8189894E-1220.848478145
TAGATCG7150.020.498573145
TGACGCG7600.020.299833145
TGTACCG2057.038584E-419.262455
CCGACGG2600.001195473817.801394145
TAGGCCT2601.6958192E-417.7189854
ACTATGC5352.6011548E-1017.222198
CGCGTGG8200.016.933031145
GGTGGTG15700.016.705341145
GCCGACC10700.016.58136145
TAGATTG6751.8189894E-1216.5752185
GTATTAG8000.016.4561651
Produced by FastQC (version 0.11.5)