FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_msh3_MA_strain_A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_msh3_MA_strain_A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869402
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCT1456.785376E-527.212015
CGGTGGG9200.026.843666145
GCCGACC7300.025.37278145
TGACGCG6100.025.303457145
TCTCGGG7450.023.826006145
TAGATCG4952.055458E-1020.268326145
GCGTGTG3159.816109E-619.600138145
TACGCGG7500.019.551138145
GCTGCCC6007.2759576E-1219.293884145
CCTGAGG2250.001321547117.5376363
CTATACT7900.017.4811334
TCTGACC5751.5152182E-917.448383145
CCTGTCG3152.1757363E-417.150122145
ACGATCG6353.092282E-1017.015081145
TGTCGGG3202.4537693E-416.882149145
TCGCCGG11900.016.861883145
CCTGAAC3153.8889237E-516.702513
CGCGTGG6254.5874913E-916.052513145
GTATATA24600.016.043291
ATACCGT4201.6455742E-615.6577026
Produced by FastQC (version 0.11.5)