FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_Federica.Wine.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_Federica.Wine.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264121
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1205.886579E-638.88714145
ACTCTGG1806.931987E-834.56635145
CACATCG1502.7141477E-531.10971145
GCCGACC3653.6379788E-1227.70043145
TCTCGGG2451.0312942E-625.395685145
CTACTCG1951.6193927E-423.93055145
TCGCCGG3952.419256E-1023.62763145
CGACGAG1700.002052064522.87479145
GCTGCCC2254.26391E-420.739807145
TTCTAGC1600.0040722520.3281232
CAGAAGT1600.00407671920.3242744
TAGTGTA1650.00487134519.7083855
TATCCTA1650.00487134519.7083855
CTACAAC2150.001045821118.1534863
TACCCTT1800.008050919518.0660215
ACTGCAT1800.008050919518.0660218
GTACTAT1850.00941722917.581081
GTATATA6700.017.4761161
GTATTAC2300.00164351616.9695631
AATACTT3908.469615E-716.6763275
Produced by FastQC (version 0.11.5)