FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_Federica.Oak.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_Federica.Oak.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201941
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1001.6758222E-646.672215145
GAACCAG608.420948E-443.367026
AGGAGTC750.002521137534.693627
ACATCCC850.00465419430.6120178
CGGAACT850.00465983930.6044379
ACGGAAC900.00615450428.9113468
ACAGAGC900.00615450428.9113468
GCTGCCC1355.33735E-428.81001145
ACTGTCG1100.00684482128.286192145
ACCTACC950.00801363427.3896988
TTCCGGG1350.001516959324.092792
GTCTAAA1853.7975438E-421.0974711
CTTGTAA1600.004074624720.3232569
TATTGTG1700.005777634719.1325117
ATACAAG2108.9190545E-418.5858651
CAATACC2300.001641612216.9697044
ATAATAG5605.7843863E-1016.2626343
AAGATAG2450.002504007415.9307425
TATTATG4252.0654297E-615.3060082
TATATTC4804.451631E-714.90741353
Produced by FastQC (version 0.11.5)