FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_Federica.F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_Federica.F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530256
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1250.061.038567145
CGGTGGG4001.2732926E-1124.796917145
GACTCCG4354.1836756E-1122.80176145
ACCGTCT1802.6259077E-422.290828
GCCGACC2803.9057704E-621.799486145
TAGATCG2453.4448738E-521.799486145
GTACCGA1600.003472547320.8996146
CGTACGG1850.003752545220.621136145
CTGGGAC1650.0041501420.266293
GGCGGAT1900.004379153320.078474145
ACAGTGT3351.287899E-719.9619268
GAGAGTG1700.004932689519.6702237
TAGACTG2459.500635E-519.1082175
GCTGCCC3251.4167343E-518.781096145
ACTGTGC2158.702581E-418.662088
ATACAGG4809.1858965E-1018.1129996
TAGACAG3002.212369E-517.8343355
CCTTGCG1900.00937726817.5980139
GTATTAG6155.456968E-1217.4009931
TTAGACT3102.9322895E-517.2590354
Produced by FastQC (version 0.11.5)