FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_Federica.CSS_parent.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_Federica.CSS_parent.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6562032
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG32550.037.470825145
TCTCGGG61650.031.977695145
CGGTGGG77900.030.427633145
GCTGCCC57500.029.082771145
TAGATCG45650.025.37367145
CTTACGG59100.025.310125145
GCCGACC65000.024.900969145
TCTGACC51600.021.704525145
GACGACG90100.021.029034145
TCGCCGG119850.018.49724145
CGCCGTG31550.017.992145
CACATCG45550.017.514273145
GACGACC20600.017.5016145
GACTCCG96900.017.415905145
CGTACGG23550.016.612171145
CCGACGC223000.016.442587145
ACGTGTT46500.015.011897145
CCTGTCG31000.014.8469305145
GGTGGTG94000.014.362562145
GTGTAGG27400.014.277979145
Produced by FastQC (version 0.11.5)