FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n02_Federica.A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n02_Federica.A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502401
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1900.049.319477145
TCTCGGG3151.2732926E-1129.748257145
CACATCG2356.9459566E-726.583548145
GCTGCCC3907.2759576E-1226.029724145
ACTCTGG4101.4551915E-1124.759983145
CTACTCG4053.1650416E-1023.137533145
ACTCACC1400.001944025723.095578
ATACGGG1450.00238246122.301396
TCTGACC3707.145354E-821.105183145
GCCGCCC2604.5533106E-521.024008145
CATAGAC2807.016133E-720.7901523
CTGTACT2807.022845E-720.7880824
GGCTTAT2205.3653886E-520.58221
TCTAGGC1650.00503519819.6001422
CCGACGC6751.8189894E-1218.510027145
CCGCTAT1750.007081342418.4764589
GTGTAGG2150.00782036818.16027145
AATACGG2851.8984267E-518.1541145
TAATACG3654.5087472E-717.7189124
CGGTGGG5901.8189894E-917.206089145
Produced by FastQC (version 0.11.5)