FastQCFastQC Report
Wed 16 Dec 2020
HK3TCAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3TCAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9469791
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG62400.010.7869649
GTTCCGG125550.010.5544311
ATACAGG75700.010.5191416
GTATATA288000.09.6547091
TATATAC278550.09.6417123
ATAGTCC82500.09.4748148
TACAGGC43950.09.3868357
TTCCGGG119300.09.1019462
GAGCCCC142200.09.086868145
CCGGCTC39650.08.9461544
TATACTG99150.08.90739255
AGTTCCG97950.08.6453458
TATACGG59500.08.6382315
CCCTTAT44000.08.5575831
TATACCG47650.08.5076295
ACTATGC59600.08.5006618
TCTATAC70900.08.4745243
TACTATG83150.08.3562647
CACATCG64400.08.3414135145
CCTATAC50450.08.3224323
Produced by FastQC (version 0.11.5)